Kevin A. Roth, M.D., Ph.D., the Editor-In-Chief of the Journal of Histochemistry and Cytochemistry and the Chairman of the Pathology Dept at UAB, is teaching the Short Course session on Amplification Medthods. He has agreed to lead the following laboratory session:
A hands on comparison of standard fluorescence immunohistochemical detection versus tyramide signal amplification (TSA) and TSA Plus detection will allow Short Course attendees to gain experience with these sensitve detection techniques. Helpful troubleshooting tips and in depth discussion of TSA procedures with experts in this technique will provide attendees opportunity to fully utilize modern amplification techniques in their research.
Dr. Roth was selected Lecturer of the Year three times and received seven Distinguished Teaching Service Awards when he taught at Washington University. You don’t want to miss this session!
Categories: Hands on Lab Session · immunohistochemistry · microscopy
The Histochemical Society’s annual meeting, New Trends in Microscopy and Immunohistochemistry is March 21-23, 2010 and precedes The HCS Annual Short Course which has sort of the reverse title. Don’t get confused! HCS is offering a 15% discount for those attending both the meeting and the short course.
The meeting theme is Three -Dimensional imaging Technologies: Bridging the Gap between Light and Electron Microscopy. The meeting organizer’s are Eduardo Rosa-Molinar of the University of Puerto Rico and Mark Sanders of the University of Minnesota.
Registration for both the meeting and the short course can be found here.
Both registration packages include three nights lodge and full meal package.
Categories: Meetings · immunohistochemistry · microscopy
Tagged: immunohistochemistry, microscopy
All the faculty of Principles and Applications of Immunocytochemistry have published articles which focus on their particular presentations. Below you will find links to articles which faculty members have chosen as representative of their work.
Richard W. Burry
Richard W. Burry Specificity Controls for Immunocytochemical Methods J. Histochem. Cytochem., 48: 163-166, 2000. -
Richard W. Burry and Catherine L. Smith
HuD Distribution changes in Response to Heat Shock but Not Neurotrophic Stimulation
J. Histochem. Cytochem. 54:1129-1138, 2006.
Kevin A. Roth
Shindler KS, Roth KA: Double immunofluorescent staining using two unconjugated primary antisera raised in the same species. J. Histochem. Cytochem. 1996; 44:1331-1335. PubMed ID: 8918908
Zaidi AU, Enomoto H, Milbrandt J, Roth KA: Dual fluorescent in situ hybridization and immunohistochemistry with tyramide signal amplification. J. Histochem. Cytochem. 2000; 48:1369-1376. PubMed ID: 10990490
Ness JM, Akhtar RS, Latham CB, Roth KA: Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence. J. Histochem. Cytochem., 2003; 51:981-987. PubMed ID: 12871979
Roth KA: A Beautiful Science. J. Histochem. Cytochem. 2006; 54:1073-1074. PubMed ID: 16980508
Categories: Articles
Tagged: controls, immunocytochemistry, immunofluorescent staining, Q dots
Bill Stahl on Immunocytochemistry
I have been using immunocytochemical techniques in my research for over thirty years.
I sometimes get asked what is immunocytochemistry and what do you do with it? Immunocytochemistry is the process of localizing antigens in biological tissues and cells using target specific antibodies. More formally immunohistochemistry refers to the process as applied to tissues and immunocytochemistry refers to localization in cells but the terms are often used interchangeably. The most typical sites identified by immunocytochemistry are proteins, but lipids and other cellular constituents can also be localized both by light, fluorescence and electron microscopy. Applications extend from research in cell biology to localization of specific markers in specimens sent to the pathology lab to support clinical diagnoses
Almost all biologists utilize ICC in their work.
It’s an extremely useful scientific method for research but it can be misunderstood in its implementation and interpretation. It’s easy to purchase a commercial kit to carry out an immunocytochemistry application but a basic understanding of underlying principles and potential problems is essential if you want to be successful in your use of ICC. It also helps to get your papers published and your research grants funded.
Categories: Immunocytochem
Tagged: course, immunocytochemistry, immunohistochemistry
